markw

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markw
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  • johnsonko

    Hello, Need more information regarding the .bam file output please. Is this an unaligned bam of reads bearing kmers having match the microbes detailed in the output .txt file or is it a bam file containing kmer aligned reads to the reference sequence described in the output .txt file. Would like to know if I am to import the entire reference.fa file into a viewer like IGV to see where the reads fall on the reference or is the purpose of the output bam for me to grab those annotated as matching a microbe of interest and me doing de novo assembly and blast of the contig and/or directed alignment against the microbe reference sequence? Thank you!

    September 7
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    August 18