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BD and BI tags
hansvanvliet
Member ✭
After Fixmating(Picard) my Bam file I BaseRecalibrated the new Bam file.
Its size jumped from 101 to 173 Gb.
Is this due to the added BD and BI tags?
What is the meaning of these tags?
Are they used in variant calling?
Tagged:
Best Answers
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Geraldine_VdAuwera
Cambridge, MA admin
Hi Hans,
These are base insertion and deletion qualities. See this thread for a little more background info.
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Geraldine_VdAuwera
Cambridge, MA admin
Hi Hans,
To clarify, the variant callers (both UG and HC) still use the BD and BI tags for calling. We are still looking into other approaches. My apologies if the information I linked to was confusing.
Answers
Hi Hans,
These are base insertion and deletion qualities. See this thread for a little more background info.
Thanks again.
I understand that these BD and BI tags are not used in further analysis of the Bam data such as variant calling etc.
Hi Hans,
To clarify, the variant callers (both UG and HC) still use the BD and BI tags for calling. We are still looking into other approaches. My apologies if the information I linked to was confusing.
Thanks again Geraldine. Looking at your name we could be 'zuiderburen'.
Hah, indeed; I'm a recently transplanted Brussels sprout.
Hi Geraldine,
Just checking if GATK3.1.x variant callers are still using BD and BI tags? It's been about 6 months since the last comment here.
@tgenahmet I believe they still are.