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GermlineCNVCaller Stderr

Hi,

I ran this command line with the counts having been generated by CollectReadCounts and the contig-ploidy-calls file being generated by DetermineGermlineContigPloidy:

```
gatk GermlineCNVCaller \
--run-mode COHORT \
--contig-ploidy-calls results/190225.181217_K00178.ploidy/cohort-calls/ \
--input results/200219_X008378.counts.tsv \
--input results/200219_X008388.counts.tsv \
--input results/200219_X008389.counts.tsv \
--input results/200219_X008409.counts.tsv \
--input results/200219_X008410.counts.tsv \
--input results/200219_X008411.counts.tsv \
--input results/200219_X008412.counts.tsv \
--input results/200219_X008415.counts.tsv \
--input results/200219_X008417.counts.tsv \
--input results/200219_X008420.counts.tsv \
--input results/200219_X008422.counts.tsv \
--input results/200219_X008423.counts.tsv \
--input results/200219_X008429.counts.tsv \
--input results/200219_X008430.counts.tsv \
--input results/200219_X008432.counts.tsv \
--input results/200219_X008458.counts.tsv \
--input results/200219_X008493.counts.tsv \
--input results/200219_X008504.counts.tsv \
--input results/200219_X008512.counts.tsv \
--input results/200219_X008522.counts.tsv \
--input results/200219_X008523.counts.tsv \
--input results/200219_X008525.counts.tsv \
--input results/200219_X008528.counts.tsv \
--input results/200219_X008543.counts.tsv \
--output results/190225.181217_K00178.CNVCaller \
--output-prefix cohort
```

but i encountered this error:

```
[February 25, 2019 3:59:35 PM GMT] org.broadinstitute.hellbender.tools.copynumber.GermlineCNVCaller done. Elapsed time: 106.19 minutes.
Runtime.totalMemory()=745537536
org.broadinstitute.hellbender.utils.python.PythonScriptExecutorException:
python exited with 137
Command Line: python /tmp/cohort_denoising_calling.5199478827672377510.py --ploidy_calls_path=/mnt/storage/home/kimy/projects/CNV_calling/results/190225.181217_K00178.ploidy/cohort-calls --output_calls_path=/mnt/storage/home/kimy/projects/CNV_calling/results/190225.181217_K00178.CNVCaller/cohort-calls --output_tracking_path=/mnt/storage/home/kimy/projects/CNV_calling/results/190225.181217_K00178.CNVCaller/cohort-tracking --modeling_interval_list=/tmp/intervals6808861825847823427.tsv --output_model_path=/mnt/storage/home/kimy/projects/CNV_calling/results/190225.181217_K00178.CNVCaller/cohort-model --enable_explicit_gc_bias_modeling=False --read_count_tsv_files /tmp/sample-03370867836644351516.tsv /tmp/sample-11290385693458589644.tsv /tmp/sample-26680414097017365398.tsv /tmp/sample-34861302842039719789.tsv /tmp/sample-46167771232071802189.tsv /tmp/sample-5397722415827930713.tsv /tmp/sample-68905563219726535027.tsv /tmp/sample-71822298944654562942.tsv /tmp/sample-82852469154924336573.tsv /tmp/sample-94435864773747747133.tsv /tmp/sample-10291209000691874035.tsv /tmp/sample-112960128087988103802.tsv /tmp/sample-125642393799602752840.tsv /tmp/sample-137913221119280339269.tsv /tmp/sample-148798104107874397064.tsv /tmp/sample-151707670833440725046.tsv /tmp/sample-165190254391986506906.tsv /tmp/sample-171669462045718151420.tsv /tmp/sample-1886514412161155023.tsv /tmp/sample-197025230919736630167.tsv /tmp/sample-202669755366650912708.tsv /tmp/sample-218211154753873225756.tsv /tmp/sample-227491296710974059874.tsv /tmp/sample-235757810671885317124.tsv --psi_s_scale=1.000000e-04 --mapping_error_rate=1.000000e-02 --depth_correction_tau=1.000000e+04 --q_c_expectation_mode=hybrid --max_bias_factors=5 --psi_t_scale=1.000000e-03 --log_mean_bias_std=1.000000e-01 --init_ard_rel_unexplained_variance=1.000000e-01 --num_gc_bins=20 --gc_curve_sd=1.000000e+00 --active_class_padding_hybrid_mode=50000 --enable_bias_factors=True --disable_bias_factors_in_active_class=False --p_alt=1.000000e-06 --cnv_coherence_length=1.000000e+04 --max_copy_number=5 --p_active=0.010000 --class_coherence_length=10000.000000 --learning_rate=1.000000e-02 --adamax_beta1=9.000000e-01 --adamax_beta2=9.900000e-01 --log_emission_samples_per_round=50 --log_emission_sampling_rounds=10 --log_emission_sampling_median_rel_error=5.000000e-03 --max_advi_iter_first_epoch=5000 --max_advi_iter_subsequent_epochs=200 --min_training_epochs=10 --max_training_epochs=50 --initial_temperature=1.500000e+00 --num_thermal_advi_iters=2500 --convergence_snr_averaging_window=500 --convergence_snr_trigger_threshold=1.000000e-01 --convergence_snr_countdown_window=10 --max_calling_iters=10 --caller_update_convergence_threshold=1.000000e-03 --caller_internal_admixing_rate=7.500000e-01 --caller_external_admixing_rate=1.000000e+00 --disable_caller=false --disable_sampler=false --disable_annealing=false
Stdout: 14:13:50.032 INFO cohort_denoising_calling - Loading 24 read counts file(s)...
14:13:53.719 INFO gcnvkernel.io.io_metadata - Loading germline contig ploidy and global read depth metadata...
14:13:58.626 INFO gcnvkernel.tasks.task_cohort_denoising_calling - Instantiating the denoising model (warm-up)...
14:14:04.543 INFO gcnvkernel.models.fancy_model - Global model variables: {'W_tu', 'psi_t_log__', 'ard_u_log__', 'log_mean_bias_t'}
14:14:04.544 INFO gcnvkernel.models.fancy_model - Sample-specific model variables: {'z_su', 'psi_s_log__', 'read_depth_s_log__'}
14:14:04.544 WARNING gcnvkernel.tasks.inference_task_base - No log emission sampler given; skipping the sampling step
14:14:04.544 WARNING gcnvkernel.tasks.inference_task_base - No caller given; skipping the calling step
14:14:04.544 INFO gcnvkernel.tasks.inference_task_base - Instantiating the convergence tracker...
14:14:04.544 INFO gcnvkernel.tasks.inference_task_base - Setting up DA-ADVI...
14:14:10.902 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up)) starting...: 0%| | 0/5000 [00:00<?, ?it/s]
14:14:12.877 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: N/A, SNR: N/A, T: 1.50: 0%| | 1/5000 [00:01<2:44:32, 1.97s/it]
14:14:14.753 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -145.294 +/- 0.000, SNR: 35869952999211676.0, T: 1.50: 0%| | 2/5000 [00:03<2:40:21, 1.93s/it]
14:14:16.609 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -110.174 +/- 10.941, SNR: 568.3, T: 1.50: 0%| | 3/5000 [00:05<2:38:25, 1.90s/it]
14:14:18.444 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -125.004 +/- 11.232, SNR: 292.3, T: 1.50: 0%| | 4/5000 [00:07<2:36:59, 1.89s/it]
14:14:20.289 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -144.050 +/- 15.199, SNR: 8.9, T: 1.50: 0%| | 5/5000 [00:09<2:36:16, 1.88s/it]
14:14:22.116 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -144.733 +/- 13.469, SNR: 1.3, T: 1.50: 0%| | 6/5000 [00:11<2:35:33, 1.87s/it]
14:14:23.926 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -147.237 +/- 12.420, SNR: 24.0, T: 1.50: 0%| | 7/5000 [00:13<2:34:49, 1.86s/it]
14:14:25.759 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -150.242 +/- 11.819, SNR: 53.3, T: 1.50: 0%| | 8/5000 [00:14<2:34:30, 1.86s/it]
14:14:27.583 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.414 +/- 11.116, SNR: 41.3, T: 1.50: 0%| | 9/5000 [00:16<2:34:10, 1.85s/it]
14:14:29.518 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -149.789 +/- 10.551, SNR: 55.2, T: 1.50: 0%| | 10/5000 [00:18<2:34:49, 1.86s/it]
14:14:31.282 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -150.978 +/- 10.081, SNR: 67.5, T: 1.50: 0%| | 11/5000 [00:20<2:34:02, 1.85s/it]
14:14:33.089 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -147.531 +/- 10.426, SNR: 41.0, T: 1.50: 0%| | 12/5000 [00:22<2:33:42, 1.85s/it]
14:14:34.882 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.847 +/- 10.066, SNR: 51.5, T: 1.50: 0%| | 13/5000 [00:23<2:33:18, 1.84s/it]
14:14:36.873 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.074 +/- 9.669, SNR: 48.5, T: 1.50: 0%| | 14/5000 [00:25<2:34:09, 1.86s/it]
14:14:38.734 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -149.072 +/- 9.380, SNR: 56.4, T: 1.50: 0%| | 15/5000 [00:27<2:34:09, 1.86s/it]
14:14:40.604 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -149.567 +/- 9.052, SNR: 61.6, T: 1.50: 0%| | 16/5000 [00:29<2:34:11, 1.86s/it]
14:14:42.485 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.402 +/- 8.903, SNR: 55.7, T: 1.50: 0%| | 17/5000 [00:31<2:34:17, 1.86s/it]
14:14:44.272 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -149.207 +/- 8.697, SNR: 61.7, T: 1.50: 0%| | 18/5000 [00:33<2:33:56, 1.85s/it]
14:14:46.178 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -149.406 +/- 8.426, SNR: 64.8, T: 1.50: 0%| | 19/5000 [00:35<2:34:07, 1.86s/it]
14:14:48.136 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -147.117 +/- 9.040, SNR: 48.9, T: 1.50: 0%| | 20/5000 [00:37<2:34:31, 1.86s/it]
14:14:50.017 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -146.929 +/- 8.773, SNR: 49.4, T: 1.50: 0%| | 21/5000 [00:39<2:34:33, 1.86s/it]
14:14:51.976 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -147.356 +/- 8.553, SNR: 52.7, T: 1.50: 0%| | 22/5000 [00:41<2:34:53, 1.87s/it]
14:14:53.981 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.649 +/- 8.651, SNR: 58.1, T: 1.50: 0%| | 23/5000 [00:43<2:35:21, 1.87s/it]
14:14:55.909 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.848 +/- 8.430, SNR: 60.5, T: 1.50: 0%| | 24/5000 [00:45<2:35:31, 1.88s/it]
14:14:57.761 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -148.098 +/- 8.342, SNR: 57.8, T: 1.50: 0%| | 25/5000 [00:46<2:35:24, 1.87s/it]
14:14:59.742 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -147.870 +/- 8.153, SNR: 58.2, T: 1.49: 1%| | 26/5000 [00:48<2:35:43, 1.88s/it]
14:15:01.759 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -147.645 +/- 7.977, SNR: 58.5, T: 1.49: 1%| | 27/5000 [00:50<2:36:07, 1.88s/it]
14:15:03.674 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -146.458 +/- 8.181, SNR: 52.3, T: 1.49: 1%| | 28/5000 [00:52<2:36:10, 1.88s/it]
14:15:05.669 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -145.601 +/- 8.211, SNR: 48.7, T: 1.49: 1%| | 29/5000 [00:54<2:36:27, 1.89s/it]
14:15:07.503 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -144.395 +/- 8.461, SNR: 42.9, T: 1.49: 1%| | 30/5000 [00:56<2:36:16, 1.89s/it]
14:15:09.330 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -143.653 +/- 8.457, SNR: 40.3, T: 1.49: 1%| | 31/5000 [00:58<2:36:05, 1.88s/it]
14:15:11.195 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -143.713 +/- 8.287, SNR: 41.3, T: 1.49: 1%| | 32/5000 [01:00<2:36:00, 1.88s/it]
14:15:13.165 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -144.466 +/- 8.290, SNR: 43.8, T: 1.49: 1%| | 33/5000 [01:02<2:36:11, 1.89s/it]
14:15:15.037 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -144.273 +/- 8.147, SNR: 43.9, T: 1.49: 1%| | 34/5000 [01:04<2:36:07, 1.89s/it]
14:15:17.018 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -143.297 +/- 8.331, SNR: 39.8, T: 1.49: 1%| | 35/5000 [01:06<2:36:18, 1.89s/it]
14:15:18.990 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -143.084 +/- 8.201, SNR: 39.7, T: 1.49: 1%| | 36/5000 [01:08<2:36:28, 1.89s/it]
14:15:20.885 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -143.530 +/- 8.123, SNR: 41.4, T: 1.49: 1%| | 37/5000 [01:09<2:36:27, 1.89s/it]
14:15:22.790 INFO gcnvkernel.tasks.inference_task_base - (denoising (warm-up) epoch 1) ELBO: -143.881 +/- 8.026, SNR: 43.0, T: 1.49: 1%| | 38/5000 [01:11<2:36:26, 1.89s/it]
14:15:24.618 INFO gcnvkernel.tasks.inference_task_base - (denoisi
Stderr:
at org.broadinstitute.hellbender.utils.python.PythonExecutorBase.getScriptException(PythonExecutorBase.java:75)
at org.broadinstitute.hellbender.utils.runtime.ScriptExecutor.executeCuratedArgs(ScriptExecutor.java:126)
at org.broadinstitute.hellbender.utils.python.PythonScriptExecutor.executeArgs(PythonScriptExecutor.java:170)
at org.broadinstitute.hellbender.utils.python.PythonScriptExecutor.executeScript(PythonScriptExecutor.java:151)
at org.broadinstitute.hellbender.utils.python.PythonScriptExecutor.executeScript(PythonScriptExecutor.java:121)
at org.broadinstitute.hellbender.tools.copynumber.GermlineCNVCaller.executeGermlineCNVCallerPythonScript(GermlineCNVCaller.java:441)
at org.broadinstitute.hellbender.tools.copynumber.GermlineCNVCaller.doWork(GermlineCNVCaller.java:288)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:138)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:191)
at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:210)
at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:162)
at org.broadinstitute.hellbender.Main.mainEntry(Main.java:205)
at org.broadinstitute.hellbender.Main.main(Main.java:291)
Using GATK jar /mnt/storage/apps/software/gatk/4.1.0.0/gatk-package-4.1.0.0-local.jar
Running:
java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /mnt/storage/apps/software/gatk/4.1.0.0/gatk-package-4.1.0.0-local.jar GermlineCNVCaller --run-mode COHORT --contig-ploidy-calls results/190225.181217_K00178.ploidy/cohort-calls/ --input results/200219_X008378.counts.tsv --input results/200219_X008388.counts.tsv --input results/200219_X008389.counts.tsv --input results/200219_X008409.counts.tsv --input results/200219_X008410.counts.tsv --input results/200219_X008411.counts.tsv --input results/200219_X008412.counts.tsv --input results/200219_X008415.counts.tsv --input results/200219_X008417.counts.tsv --input results/200219_X008420.counts.tsv --input results/200219_X008422.counts.tsv --input results/200219_X008423.counts.tsv --input results/200219_X008429.counts.tsv --input results/200219_X008430.counts.tsv --input results/200219_X008432.counts.tsv --input results/200219_X008458.counts.tsv --input results/200219_X008493.counts.tsv --input results/200219_X008504.counts.tsv --input results/200219_X008512.counts.tsv --input results/200219_X008522.counts.tsv --input results/200219_X008523.counts.tsv --input results/200219_X008525.counts.tsv --input results/200219_X008528.counts.tsv --input results/200219_X008543.counts.tsv --output results/190225.181217_K00178.CNVCaller --output-prefix cohort
```

I found other threads having a similar issue but using DetermineGermlineContigPloidy, can anyone help?

Answers

  • shleeshlee CambridgeMember, Broadie ✭✭✭✭✭

    Hi @kimy,

    I think this is related to the GitHub issue at https://github.com/broadinstitute/gatk/issues/4825. Can you check to see if your output file paths already exist?

    --output results/190225.181217_K00178.CNVCaller \
    --output-prefix cohort
    

    The workflow expects to write fresh ones and will not overwrite existing file paths. If you could confirm for us that this was indeed the issue, then I can put in a request for better handling, i.e. a check before the analysis begins and a better error message. Feel free to add your two cents to the issue ticket as well.

  • kimykimy Member
    Yes, I created the folder because when I first ran it it gave me this error:

    08:37:16.407 INFO GermlineCNVCaller - Start Date/Time: February 26, 2019 8:37:04 AM GMT
    08:37:16.407 INFO GermlineCNVCaller - ------------------------------------------------------------
    08:37:16.407 INFO GermlineCNVCaller - ------------------------------------------------------------
    08:37:16.408 INFO GermlineCNVCaller - HTSJDK Version: 2.18.2
    08:37:16.408 INFO GermlineCNVCaller - Picard Version: 2.18.25
    08:37:16.408 INFO GermlineCNVCaller - HTSJDK Defaults.COMPRESSION_LEVEL : 2
    08:37:16.408 INFO GermlineCNVCaller - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false
    08:37:16.408 INFO GermlineCNVCaller - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true
    08:37:16.408 INFO GermlineCNVCaller - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false
    08:37:16.408 INFO GermlineCNVCaller - Deflater: IntelDeflater
    08:37:16.409 INFO GermlineCNVCaller - Inflater: IntelInflater
    08:37:16.409 INFO GermlineCNVCaller - GCS max retries/reopens: 20
    08:37:16.409 INFO GermlineCNVCaller - Requester pays: disabled
    08:37:16.409 INFO GermlineCNVCaller - Initializing engine
    08:37:21.698 INFO GermlineCNVCaller - Done initializing engine
    08:37:22.015 INFO GermlineCNVCaller - Retrieving intervals from read-count file (results/200219_X008378.counts.tsv)...
    08:37:22.119 INFO GermlineCNVCaller - No annotated intervals were provided...
    08:37:22.120 INFO GermlineCNVCaller - No GC-content annotations for intervals found; explicit GC-bias correction will not be performed...
    08:37:22.194 INFO GermlineCNVCaller - Running the tool in the COHORT mode...
    08:37:22.195 INFO GermlineCNVCaller - Shutting down engine
    [February 26, 2019 8:37:22 AM GMT] org.broadinstitute.hellbender.tools.copynumber.GermlineCNVCaller done. Elapsed time: 0.29 minutes.
    Runtime.totalMemory()=330301440
    java.lang.IllegalArgumentException: Output directory results/190226.181217_K00178.CNVCaller does not exist.
    at org.broadinstitute.hellbender.utils.Utils.validateArg(Utils.java:724)
    at org.broadinstitute.hellbender.tools.copynumber.GermlineCNVCaller.validateArguments(GermlineCNVCaller.java:361)
    at org.broadinstitute.hellbender.tools.copynumber.GermlineCNVCaller.doWork(GermlineCNVCaller.java:281)
    at org.broadinstitute.hellbender.cmdline.CommandLineProgram.runTool(CommandLineProgram.java:138)
    at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMainPostParseArgs(CommandLineProgram.java:191)
    at org.broadinstitute.hellbender.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:210)
    at org.broadinstitute.hellbender.Main.runCommandLineProgram(Main.java:162)
    at org.broadinstitute.hellbender.Main.mainEntry(Main.java:205)
    at org.broadinstitute.hellbender.Main.main(Main.java:291)
    Using GATK jar /mnt/storage/apps/software/gatk/4.1.0.0/gatk-package-4.1.0.0-local.jar
    Running:
    java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -jar /mnt/storage/apps/software/gatk/4.1.0.0/gatk-package-4.1.0.0-loc
  • sleeslee Member, Broadie, Dev ✭✭✭

    @kimy I responded to you over in the issue thread.

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