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*bam files with the same size (# of contigs) to merge?


I am trying to merge multiple (4 files data fom UCEs) *bam files using MergeSamFiles.jar. However, the script shows this message:

Exception in thread "main" net.sf.samtools.util.SequenceUtil$SequenceListsDifferException: Sequence dictionaries are not the same size (3857, 3743)

Indeed, the *bam files have different sizes (and their dictionaries), but my experiment dont allow edit the files to be equal in size. I use the samtools view -H command(s) to see the format and information of each file, so I think that that is not the problem.

So, i have a question:

  • It is necessary that the *bam/sam files have the same size (contigs) to be merged?

Sorry by this basic question, but I dont find information on this.

Thanks in advance.


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