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problem using -L and -XL option in PrintReads
Dear GATK team,
I am using the -L and -XL options together in my PrintReads command to only print reads in regions of interest and to exclude reads in Blacklisted regions.
Below is an example command:
java -Xmx4g -jar GenomeAnalysisTK.jar -T PrintReads -R GRCh37-lite.fa -L 1:1-61913548 -XL all_Enhancers.intervals -XL Blacklist_merged.intervals -I input.bam -nct 8 -BQSR recal_data.wg.table -o output.bam
However, I've noticed that the output.bam has reads that are in intervals of my Blacklist file. For example,
HWI-ST1133:217:D1D4WACXX:8:1206:15633:92032 163 1 19022 10 101M = 19168 247 TCCCCAGACATCCCTGTGGCTGGCTCCTGATGCCCGAGGCCCAAGTGTCTGATGCTTTAAGGCACATCACCCCACTCATGCTTTTCCATGTTCTTTGGCCC 249<ADCDEEEDAADEDABDCEBDDEBEBDFBEBB4B:@CDBCA?FFHFE>DGGDGBBCCDCEFDEE?GEFFGGCHB=EAHIDJJ?M=GAHEGHKD<=@D# X0:i:7 X1:i:0 MC:Z:101M BD:Z:IIMJKNOLLHLLLILMHGMMMMMMMJLLMMKNNNJKLMNNNJNJLNHINKNNLNNNJCKKLNNNHIMMLHNKKOINLMNOOOKDDMOPOPKNONNGRNNNI MD:Z:100G0 RG:Z:0.2 XG:i:0 BI:Z:LLOLLQQMOKOONJNOJINNPPOOPKNOPPOPPOKMONOPPLQMOQKLPNQQPQQQMFOMOOPPKMQQPLPMMRLQMPQRRRNGHQQSRSOPRQPJTPPPL AM:i:0 NM:i:1 SM:i:0 XM:i:1 XO:i:0 MQ:i:18 XT:A:R
My Blacklist file includes the interval: 1:18906-19049, so I thought the above read would not show up in my output .bam.
Do you know where I've made a mistake, and is there a better way to exclude reads in Blacklist regions?
Thanks a lot for your help!