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If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!
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Error when run FastaAlternateReferenceMake

I try to run this command java -jar GenomeAnalysisTK.jar \
-T FastaAlternateReferenceMaker \
-R reference.fasta \
-o output.fasta \
-V input.vcf \
And I get the error MESSAGE: I/O error loading or writing tribble index file for..........
I have two questions, one is why the error occur , another one is the output file is a vcf file ,not fasta file, any reason for that ?
Thanks
Answers
@richardshi628
Hi,
What version of GATK are you using and can you post the entire error message you get? Have a look at the tool documentation for more information: https://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_gatk_tools_walkers_fasta_FastaAlternateReferenceMaker.php
-Sheila
@richardshi628 Possibly stupid questions: Is your VCF file indexed? Do you have write permission to all directories?