This site is now read-only. You can find our new documentation site and support forum for posting questions here.
Be sure to read our welcome blog!
Picard tool AddOrReplaceReadGroups
Hi, I am analyzing NGS data from same individual which is generated from SOLiD 5500.
This time, I sequenced only 1 sample, so that I had to add at least 4 bar-cord for this one sample.
I treated generated data as one data as which was generated from one sample (1 library).
So I did sort and merge (samtools) whole data.
Then I did the AddOrReplaceReadGroups.jar (picard) and got some bam file.
However, which has no information in it (=0 bite).
I just wonder if I need to analyze those data as 4 samples.
I wonder if anyone know how I can merge whole data to generate one VCF file from this file.