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Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
VariantRecalibrator - "N" reference allele only in .recal files
I ran VariantRecalibrator and ApplyRecalibration, and everything seems to have worked fine. I just have one question: if there are no reference alleles besides "N" in my recalibrate_SNP.recal and recalibrate_INDEL.recal files, and in the "alt" field simply displays , does that mean that none of my variants were recalibrated? Just wanted to be completely sure. My original file (after running GenotypeGVCFs) has the same number of variants as the recalibrated vcf's.