The frontline support team will be slow on the forum because we are occupied with the GATK Workshop on March 21st and 22nd 2019. We will be back and more available to answer questions on the forum on March 25th 2019.
Separating reads in AD into different strands
Is there any possibility that I can separate the reads in AD (Allele Depths by sample) into 5' and 3' strands?
e.g. the current expression of AD is REF:ALT1:ALT2 ...etc,
Can I get the expression of AD like REF_5':REF_3':ALT1_5':ALT1_3':ALT2_5':ALT2_3'