Separating reads in AD into different strands
Is there any possibility that I can separate the reads in AD (Allele Depths by sample) into 5' and 3' strands?
e.g. the current expression of AD is REF:ALT1:ALT2 ...etc,
Can I get the expression of AD like REF_5':REF_3':ALT1_5':ALT1_3':ALT2_5':ALT2_3'