Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
How to split a paired-end FASTQ file into two separate FASTQ files (forward and reverse)?
I have to analyse a paired-end DNA-seq read that are in an unusual format: both pair-end reads are joined in one FASTQ. I already obtained this file by reverting from BAM file to FASTQ. So, I need to split the file in two separated FASTQ paired-end files. Any comment or suggestion would be appreciated. I know that there is a galaxy tool named FASTQ splitter that can do this for RNA-seq read but not sure this could work for DNA-seq read as well. Thanks