Our staff will be observing the holiday and will be unavailable from 22nd to 25th November. This will cause a delay in reaching out to you and answering your questions immediately. Rest assured we will get back to it on Monday November 26th. We are grateful for your support and patience.
Have a great holiday everyone!!!
Project level VCF
I am combining gVCFs that are processed exactly the same, I observed that most variants have this tag: "GT:AD:DP:GQ:PL". However for some variants, within the same variant, they can have this tag GT:AD:DP:GQ:PGT:PID:PL" for some of the samples but not others.
I did some testing
a) perform combineGVCF on a set of gVCF then do genotypeGVCF (test a)
b) perform genotypeGVCF only on this set of gVCF (test b)
What I observed is that there are some variants present only in test a, but not in test b. Same thing happens vice versa, i.e. variants present only in test b, but not in test a. These kind of variants all all got the same tag GT:AD:DP:GQ:PGT:PID:PL. Why is this the case? And what is combineGVCF and genotypeGVCF actually doing?