burden test/SKAT on exome study without control

Angel84Angel84 angelMember

hi,

We have access to exome-sequencing data from an independent set of leukemic samples from a mouse model with an induced oncogene. However, these samples do not have an associated paired (normal) control. I should come up with a pipeline for how to try to identify mutations in the absence of such paired/normal controls

I read that when we have no control in exome-seq, we should use something like 1000 genome as control and performing statistical test. if prioritization of sequence variants by tools like TAPER, PhenIX, Exomiser, FAVR, VAAST, etc, means the same and compensate for lack of controls??

thank you

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