Holiday Notice:
The Frontline Support team will be slow to respond December 17-18 due to an institute-wide retreat and offline December 22- January 1, while the institute is closed. Thank you for your patience during these next few weeks. Happy Holidays!

Mutect2 --ignore-soft-clip-bases

allieallie BostonMember

Hello,

We are running Mutect2 with the option to ignore soft clipped bases. This has significantly reduced our false positives, but the ALT and REF counts from the remaining reads do not seem to reflect what we see in IGV. Moreover, this seems to occur adjacent to the softclipped boundary. Could the softclipping index be off by 1?

Here's counts of alt and ref for one of these variants that we found:

                               ALT   REF    IGV-ALT   IGV-REF
          use softcipped       35    239     39       242
          ignore softclipped   37    235     4        237

Answers

Sign In or Register to comment.