Status for dealing with paired-end reads
Hi all, new here and its my first post, so sorry if its not relevant enough.
I have been thinking about the same problem that occurred here twice already, and i cannot seem to come to a conclusion on what do to. The problem is how to interpret paired-end reads overlapping regions. Some "commercial" software does the merging prior to any further analysis and then reports a variants coverage in relation to the merged reads. This makes sense for me, since i get a single-molecule coverage. Here in GATK as i've read in posts:
It seems that the information from the overlap is modelled (to account for varying base qualities and possible differences) but the reported coverage seems inflated. When i run the same sample through the "commercial" counterpart and GATK i get higher coverages on variants consistently.
The real problem i have with this is, that when dealing with standards and accreditations one wants to claim that variants are given for regions with coverage above 30X. But for the HaplotypeCaller, what does that mean? Should it be 60X (which would be too strict in many cases and undercut a lot of data from WES projects).
To boil it down - is there a way to bring the coverage to a value reflecting the molecules sequenced (so consider the overlap phred scores but also change the coverage to 1) or should there be some step of read-merging prior?
Sorry for a long and convoluted post, hope to hear from you!