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MarkDuplicatesWithMateCigar fails at producing metrics files with some samples

benjaminpelissiebenjaminpelissie Madison, WIMember

Hi,

With most of my samples, MarkDuplicatesWithMateCigar works just fine. For some however, while it runs for the same amount of time and seem to behave similarly, it fails at producing the metrics file and tells me that: "Exception in thread "main" picard.PicardException: Found a samRecordWithOrdinal with sufficiently large clipping that we may have missed including it in an early duplicate marking iteration. Please increase the minimum distance to at least 252bp to ensure it is considered (was 252)." What should I do?

Ben

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Answers

  • benjaminpelissiebenjaminpelissie Madison, WIMember

    Thank you @Geraldine_VdAuwera. My apologies, I didn't notice the MINIMUM_DISTANCE option in MarkDuplicatesWithMateCigar (don't know why). I'll go with MarkDuplicates anyway, as you advised me already.

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