If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!
Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
We will be out of the office on November 11th and 13th 2019, due to the U.S. holiday(Veteran's day) and due to a team event(Nov 13th). We will return to monitoring the GATK forum on November 12th and 14th respectively. Thank you for your patience.
MarkDuplicates vs. MarkduplicatesWithMateCigar: what is the best practice?
I am curious to know which tool (MarkDuplicates or MarkduplicatesWithMateCigar) would people advice for marking duplicates (I am following GATK's Best Practices from paired-end DNA reads). I get that MarkduplicatesWithMateCigar also uses CIGAR infos to mark them, but struggle justifying the supposed added value of using this tool vs. the "regular" MarkDuplicates.
Also, selecting representative nondups based on the total mapped length of a pair (ie, MarkduplicatesWithMateCigar method) rather than on the sum of base qualities of a pair (ie, Markduplicates method) seems more intuitive to me. Why would one prefer the latter over the former?