Test-drive the GATK tools and Best Practices pipelines on Terra
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How to run GATK directly on SRA files
Hello , I recently saw a webinar by NCBI "Advanced Workshop on SRA and dbGaP Data Analysis" (ftp://ftp.ncbi.nlm.nih.gov/pub/education/public_webinars/2016/03Mar23_Advanced_Workshop/). They mentioned that they were able to run GATK directly on SRA files.
I downloaded GenomeAnalysisTK-3.5 jar file to my computer. I tried both these commands:
java -jar /path/GenomeAnalysisTK-3.5/GenomeAnalysisTK.jar -T HaplotypeCaller -R SRRFileName -I SRRFileName -stand_call_conf 30 -stand_emit_conf 10 -o SRRFileName.vcf
java -jar /path/GenomeAnalysisTK-3.5/GenomeAnalysisTK.jar -T SRRFileName -R SRR1718738 -I SRRFileName -stand_call_conf 30 -stand_emit_conf 10 -o SRRFileName.vcf
For both these commands, I got this error:
ERROR MESSAGE: Invalid command line: The GATK reads argument (-I, --input_file) supports only BAM/CRAM files with the .bam/.cram extension and lists of BAM/CRAM files with the .list extension, but the file SRR1718738 has neither extension. Please ensure that your BAM/CRAM file or list of BAM/CRAM files is in the correct format, update the extension, and try again.
I don't see any documentation here about this, so wanted to check with you or anyone else has had any experience with this.