This site is now read-only. You can find our new documentation site and support forum for posting questions here.
Be sure to read our welcome blog!
Is new MQ jittering functionality recommended?
The release notes for 3.5 state "Added new MQ jittering functionality to improve how VQSR handles MQ". My understanding is that in order to use this, we will need to use the --MQCapForLogitJitterTransform argument in VariantRecalibrator. I have a few questions on this:
1) Is the above correct, i.e. the new MQ jittering functionality is only used if --MQCapForLogitJitterTransform is set to something other than the default value of zero?
2) Is the use of MQCapForLogitJitterTransform recommended?
3) If we do use MQCapForLogitJitterTransform, the tool documentation states "We recommend to either use --read-filter ReassignOriginalMQAfterIndelRealignment with HaplotypeCaller or use a MQCap=max+10 to take that into account". Is one of these to be preferred over the other? Given that it seems that sites that have been realigned can have values up to 70, but sites that have not can have values no higher than 60, it seems to me that the ReassignOriginalMQAfterIndelRealignment with HaplotypeCaller option might be preferred, but I would like to check before running.