variants filtering VQSR
I ran VQSR with 30 samples and tranche filter level of 99.0 for both SNPs and INDELs. Around 82% of my variants call pass the filter. May I know is there any standard that can be used to evaluate how good the filtering result is?
Besides, if I split the gvcfs by chromosomes and run joint genotyping (GenotypeGVCFs) at chromosome level, is it going to affect the result compared to running at whole genome level?