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Bisulfite sequencing / Cytosine methylation

Geraldine_VdAuweraGeraldine_VdAuwera Cambridge, MAMember, Administrator, Broadie admin
edited February 2016 in Dictionary

Cytosine methylation is a key component in epigenetic regulation of gene expression and frequently occurs at CpG sites throughout the genome. Bisulfite sequencing is a technique used to analyze the genome-wide methylation profiles on a single nucleotide level [doi:10.1093/nar/gki901]. Sodium bisulfite efficiently and selectively deaminates unmethylated cytosine residues to uracil without affecting 5-methyl cytosine (methylated). Using restriction enzymes and PCR to enrich for regions of the genome that have high CpG content, the resulting reduced genome comprises ~1% of the original genome but includes key regulatory sequences as well as repeated regions.

The protocol involves several steps. First, genomic DNA is digested with a restriction endonuclease such as MspI, which targets CG dinucleotides. This results in DNA fragments with CG at the ends. Next, the fragments are size selected (via gel electrophoresis), which facilitates the enrichment of CpG-containing sequences. This is followed by bisulfite treatment, which converts unmethylated C nucleotides to uracil (U) while methylated cytosines will remain intact. The bisulfite-treated DNA is amplified with a proofreading-deficient DNA polymerase to facilitate amplification of both methylated cytosines as well as the C -> U converted bases. Subsequent to PCR amplification, each original unmethylated cytosine will be converted to either a T (+ strand) or an A (- strand), while methylated C will remain a C (+ strand) or a G (- strand). The PCR products are then sequenced using conventional methods and aligned to a reference.

Post edited by dekling on
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