Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
Should RNA-seq libraries be aligned in parallel or consecutively?
I'm mapping several RNA-seq libraries to a genome with the aim of detecting nucleotide variation. I'm following the protocol "Calling variants in RNAseq".
Is it possible to do them in parallel - mapping all libraries to the genome and detecting splice junctions etc. simultaneously - or does the whole protocol need to be followed separately for each library?