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UnifiedGenotyper pooled:how to set the minimum number of reads supporting an alternate allele
I am using the UnifiedGenotyper for pooled samples and would like to set the minimum count (fraction) of reads supporting an alternate allele in order to distinguish low frequency alleles from sequencing errors. Is there a way to do it? I couldn't find a means to do that or the default threshold in the tool's documentation . Thank you for your support.