Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
Using Variant Annotator
This document is out of date and has been retired. Please see the Annotation documentation in the Tool Docs as well as various other Guide articles for better materials on annotating variants.
2 SNPs with significant strand bias
Several SNPs with excessive coverage
For a complete, detailed argument reference, refer to the GATK document page here.
In addition to true variation, variant callers emit a number of false-positives. Some of these false-positives can be detected and rejected by various statistical tests. VariantAnnotator provides a way of annotating variant calls as preparation for executing these tests.
Description of the haplotype score annotation
Examples of Available Annotations
The list below is not comprehensive. Please use the
--list argument to get a list of all possible annotations available. Also, see the FAQ article on understanding the Unified Genotyper's VCF files for a description of some of the more standard annotations.
- BaseQualityRankSumTest (BaseQRankSum)
- DepthOfCoverage (DP)
- FisherStrand (FS)
- HaplotypeScore (HaplotypeScore)
- MappingQualityRankSumTest (MQRankSum)
- MappingQualityZero (MQ0)
- QualByDepth (QD)
- ReadPositionRankSumTest (ReadPosRankSum)
- RMSMappingQuality (MQ)
- SnpEff: Add genomic annotations using the third-party tool SnpEff with VariantAnnotator
Note that technically the VariantAnnotator does not require reads (from a BAM file) to run; if no reads are provided, only those Annotations which don't use reads (e.g. Chromosome Counts) will be added. But most Annotations do require reads. When running the tool we recommend that you add the
-L argument with the variant rod to your command line for efficiency and speed.