Heads up:
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
Notice:
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!
Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
AD for 0/0 site

Hi Geraldine,
When I used UnifiedGenotyper to emit all sites, for 0/0 site, I only got GT and DP tags. Is it possible to get AD tag for these sites and get actual depth for these sites, not downsampling coverage (I can see the maximum value is 200 if I ask for downsampling to 200 when running UnifiedGenotyper)
thanks!
Answers
@ying_sheng_1
Hi,
AD cannot be annotated for 0/0 sites. In any case, it would be downsampled. The best thing to do is use DepthOfCoverage or DiagnoseTargets. Please read about them here:
https://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_gatk_tools_walkers_coverage_DepthOfCoverage.php
https://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_gatk_tools_walkers_diagnostics_diagnosetargets_DiagnoseTargets.php
You can use the VCF as the intervals list to get total depth counts at those sites.
-Sheila
Thanks!
Ying