To celebrate the release of GATK 4.0, we are giving away free credits for running the GATK4 Best Practices pipelines in FireCloud, our secure online analysis portal. It’s first come first serve, so sign up now to claim your free credits worth $250. Sponsored by Google Cloud. Learn more at

Is number of samples = 30 enough for variant calling ?

I have 90 exome samples coming from three sample groups. What would be the best idea to do variant calling using GATK? I am planning to call variant from three sample groups separately and and compare them. So, per group there are 30 samples; is this number of sample suffecient enough to merge BAM files and call variants ?

I also have RNASeq data from the same samples. Is it good idea to call variants from RNASeq data too ?


Sign In or Register to comment.