Heads up:
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
Notice:
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!

Test-drive the GATK tools and Best Practices pipelines on Terra


Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.

Fail to apply --filterExpression to raw vcf file when running VariantFiltration

liuxingliangliuxingliang SingaporeMember

Hi all,

I tried to apply the following command to my raw vcf file to filter it using the filtering expression specified in the command:

java -XX:ConcGCThreads=4 -XX:+UseConcMarkSweepGC -XX:ParallelGCThreads=4 -jar GenomeAnalysisTK.jar -T VariantFiltration -R human_g1k_v37.fa --variant human_g1k_v37.CHL124.vcf_snps.vcf -o CHL124.vcf_snps.vcf_filter_marked.vcf --filterExpression "QD < 2.0 && MQ < 40.0 && FS > 60.0 && HaplotypeScore > 13.0 && MQRankSum < -12.5 && ReadPosRankSum < -8.0" --filterName "very_small_SNPs_default_filter"

After that, I check my result file which is CHL124.vcf_snps.vcf_filter_marked.vcf, I found that, all reads are marked as "PASS" whether its QD is > 2.0 or < 2.0. Obviously, the command doesn't work, but I cannot find why everything seems goes well, no error reported.

bless~
XL

Best Answer

Answers

  • liuxingliangliuxingliang SingaporeMember

    thanks a lot, after checking the meaning of each flag, yes, each one should be filtered, so I should use OR instead of AND, thx Kurt, have a nice day:)

Sign In or Register to comment.