We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!
Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
We will be out of the office for a Broad Institute event from Dec 10th to Dec 11th 2019. We will be back to monitor the GATK forum on Dec 12th 2019. In the meantime we encourage you to help out other community members with their queries.
Thank you for your patience!
pre-processing reads and non-model BQSR
Hi! I’m trying to plan my GATK pipeline and have a few questions. We currently have an assembled genome for a non-model avian species and would like to align the reads from the single individual to the genome and subsequently mine SNPs. Our end game is to design a Fluidigm SNP chip. Since we are only working with the data from a single individual, I am trying to figure out how to best minimize false positive SNP calls.
1) Prior to beginning mapping with BWA, is any pre-processing of the reads recommended? The tutorials say to use raw reads… do we not even need to remove adaptors?
2) Does GATK have any specific recommendations for non-model BQSR (i.e., when no reference set of high-quality SNPs is available)? I’ve found a couple references to this process (http://gatkforums.broadinstitute.org/discussion/3286/quality-score-recalibration-for-non-model-organisms, https://gist.github.com/brantfaircloth/4315737). One user indicated they were using SNPs with quality scores greater than 30 as their reference SNPs for BQSR, another 90. Does GATK have any other thoughts, or is this largely a judgment call?