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VQSR VariantRecalibrator Rplot

songsysongsy University of MichiganMember

java -jar -Xmx4g GenomeAnalysisTK-2.8-1-g932cd3a/GenomeAnalysisTK.jar -T VariantRecalibrator -R hg19.fa -input NA19240.raw.SNPs.vcf -resource:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap_3.3.b37.sites.refmt.vcf -resource:omni,known=false,training=true,truth=false,prior=12.0 1000G_omni2.5.hg19.vcf -resource:dbsnp,known=true,training=false,truth=false,prior=6.0 dbsnp_138.b37.refmt.vcf -an QD -an MQ -an MQRankSum -an ReadPosRankSum -an FS -an DP -mode SNP -recalFile NA19240.raw.SNPs.recal -tranchesFile NA19240.raw.SNPs.tranches -rscriptFile NA19240.snp.plots.R

However, there is no NA19240.snp.plots.R.pdf generated. And I didn't find any error.
When I try to run NA19240.snp.plots.R in R, source('NA19240.snp.plots.R'), there is an error:
Error: Use 'theme' instead. (Defunct; last used in version 0.9.1)

How can I fix it? Thanks!!


Best Answer


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