Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
QCing IndelRealigner on low-coverage (10x) mouse WGS data
I've run the IndelRealigner on my mouse WGS *bam files with known site data from the Sanger MGP, and now I'm trying to figure out how "well" it worked.
The list created by RealignerTargetCreator contains 6547185 intervals
Parsing the output realigned.bam file for reads that had an "OC" tag added (as suggested in http://www.broadinstitute.org/gatk/events/3391/GATKw1310-BP-2-Realignment.pdf) shows that 1648299 reads were actually realigned.
I used the default settings, which means that
1) -model was USE_READS - and from what I've read, this is the correct option to use, given that Smith-Waterman modelling doesn't give greatly improved results;
2) -LOD was 5.0 - but for my data, which is mouse whole-genome sequence at average 10x coverage, this may be too high and I might be losing true positives.
I've tried randomly picking out candidate intervals from the intervals and OC-tagged reads from the realigned.bam file to check, but I was wondering if there's a more empirical way of checking how good the realignment was (I realise there's "no formal measure" as per the presentation but I'm finding it hard to make a judgement call!).
My feeling from looking at the intervals or realigned reads is that the low coverage is a major issue in terms of identifying "true" indels, so preferably we'd go for specificity over sensitivity.
Thanks for any advice/suggestions in advance!