Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
HaplotypeCaller '--emitRefConfidence' gives same PL(0) to two genotypes
In our downstream analysis we have to differentiate between non-variants because of no coverage and confident reference calls. Because of this, we need to pay close attention to HaplotypeCaller non-variant calls. Doing this we found calls like this one:
17 7127955 . G <NON_REF> . . END=7127955 GT:DP:GQ:MIN_DP:PL 0/0:29:0:29:0,0,468
Would it be possible to get a feeling why this initial call is giving the same PL(0) to 0/0 and 0/1. If you look at the alignment,after "Data Pre-processing" best practices, we can see there is strong evidence this is a 0/0 position.
GenotypeGVCFs won't call this position as variant, which is good.
PS: I tried to add a BAM file with the relevant region and I got "Uploaded file type is not allowed".