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Problem in calculating Depth of Coverage using GenomeAnalysisTK.jar
I am using this command to calculate the depth of coverage with three different formats for the EXOME_interval.list :
java -Xmx2048m -jar GenomeAnalysisTK.jar -T DepthOfCoverage -I /home/vsinha/software/bam/group1.READS.bam.list -L /home/vsinha/software/EXOME.interval_list -R /home/vsinha/software/human_g1k_v37.fasta -dt BY_SAMPLE -dcov 5000 -l INFO --omitDepthOutputAtEachBase --omitLocusTable --minBaseQuality 0 --minMappingQuality 20 --start 1 --stop 5000 --nBins 200 --includeRefNSites --countType COUNT_FRAGMENTS -o /home/vsinha/software/group1.DATA
1st format :
1 69114 69332
1 69383 69577
1 69644 69940
1 69951 70028
1 566170 566275
1 801895 801983
1 802332 802416
1 861272 861420
ERROR MESSAGE: Badly formed genome loc: Contig '1 69114 69332' does not match any contig in the GATK sequence dictionary derived from the reference; are you sure you are using the correct reference fasta file?
Badly formed genome loc: Contig '1' does not match any contig in the GATK sequence dictionary derived from the reference; are you sure you are using the correct reference fasta file?
Contig chr1 not present in sequence dictionary for merged BAM header: [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, X, Y, MT, GL000207.1, GL000226.1, GL000229.1, GL000231.1, GL000210.1, GL000239.1, GL000235.1, GL000201.1, GL000247.1, GL000245.1, GL000197.1, GL000203.1, GL000246.1, GL000249.1, GL000196.1, GL000248.1, GL000244.1, GL000238.1, GL000202.1, GL000234.1, GL000232.1, GL000206.1, GL000240.1, GL000236.1, GL000241.1, GL000243.1, GL000242.1, GL000230.1, GL000237.1, GL000233.1, GL000204.1, GL000198.1, GL000208.1, GL000191.1, GL000227.1, GL000228.1, GL000214.1, GL000221.1, GL000209.1, GL000218.1, GL000220.1, GL000213.1, GL000211.1, GL000199.1, GL000217.1, GL000216.1, GL000215.1, GL000205.1, GL000219.1, GL000224.1, GL000223.1, GL000195.1, GL000212.1, GL000222.1, GL000200.1, GL000193.1, GL000194.1, GL000225.1, GL000192.1]
Can you please let me know what is the problem in my file.
Thank You in advance.