Heads up:
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
Notice:
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!

Test-drive the GATK tools and Best Practices pipelines on Terra


Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.

what does this mean in VQSR output vcf files?

rcholicrcholic DenverMember
edited November 2013 in Ask the GATK team

I have the following entries in my vcf files output from VQSR. What does the "VQSRTrancheINDEL99.00to99.90" string mean? did they fail the recalibration?

PASS
VQSRTrancheINDEL99.00to99.90
VQSRTrancheINDEL99.00to99.90
VQSRTrancheINDEL99.00to99.90
PASS
VQSRTrancheINDEL99.00to99.90
PASS
PASS
VQSRTrancheINDEL99.90to100.00
VQSRTrancheINDEL99.90to100.00
VQSRTrancheINDEL99.90to100.00
PASS
VQSRTrancheINDEL99.00to99.90
VQSRTrancheINDEL99.00to99.90

Below is the command I used:

java -Xmx6g -jar $CLASSPATH/GenomeAnalysisTK.jar \
-T ApplyRecalibration \
-R GATK_ref/hg19.fasta \
-nt 5 \
--input ../GATK/VQSR/parallel_batch/combined_raw.snps_indels.vcf \
-mode INDEL \
--ts_filter_level 99.0 \
-recalFile ../GATK/VQSR/parallel_batch/Indels/exome.indels.vcf.recal \
-tranchesFile ../GATK/VQSR/parallel_batch/Indels/exome.indels.tranches \
-o ../GATK/VQSR/parallel_batch/Indels/exome.indels.filtered.vcf
Tagged:

Best Answer

Answers

Sign In or Register to comment.