To celebrate the release of GATK 4.0, we are giving away free credits for running the GATK4 Best Practices pipelines in FireCloud, our secure online analysis portal. It’s first come first serve, so sign up now to claim your free credits worth $250. Sponsored by Google Cloud. Learn more at https://software.broadinstitute.org/firecloud/documentation/freecredits

SelectVariants by DP >= 30

With the vcf output from GATK, I used SelectVariants to select variants with the following conditions:

java -Xmx8g -jar $CLASSPATH/GenomeAnalysisTK.jar \
-T SelectVariants \
-R GATK_ref/hg19.fasta \
-nt 5 \
-V ../GATK/VQSR/parallel_batch/Indels/exome.indels.filtered.vcf \
--excludeNonVariants \
-o ../GATK/VQSR/parallel_batch/Indels/exome.indels.filtered.selected.vcf \
-selectType INDEL \
-select "DP > 30.0"

In the output file exome.indels.filtered.selected.vcf, however, I find some variants have DP < 30, for example:

1/1:0,3:3:12:113,12,0

The bold highlighted 3 is the DP, does this mean SelectVariants did not work on my vcf?

Best Answer

Answers

Sign In or Register to comment.