Heads up:
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
Notice:
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!

Test-drive the GATK tools and Best Practices pipelines on Terra


Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.

CatVariants does not combine header

rcholicrcholic DenverMember

Below is the command:

java -cp $CLASSPATH/GenomeAnalysisTK.jar org.broadinstitute.sting.tools.CatVariants \
-R GATK_ref/hg19.fasta \
-V ../GATK/VQSR/parallel_batch/raw.snps_indels-1.vcf \
-V ../GATK/VQSR/parallel_batch/raw.snps_indels-2.vcf \
-V ../GATK/VQSR/parallel_batch/raw.snps_indels-3.vcf \
-out ../GATK/VQSR/parallel_batch/combined_raw.snps_indels.vcf \
-log ../GATK/VQSR/parallel_batch/log/combined.log \
-assumeSorted

After this, the combined_raw.snps_indels.vcf file only contains the header from raw.snps_indels-1.vcf, what might be wrong?

Best Answer

Answers

  • erikfaserikfas Member

    A related issue I had was that when I was trying to concatenate two VCFs from the same sample, one containing the (filtered) variants and one with non-variants, I got an error saying that the FS filter field wasn't in the header. This was because I had set the non-filtered VCF as first input, making the script take the header from that file, which of course didn't have a FS filter field (because no VariantFiltration had been run on it). It was easily solved by just reversing the input ordering, making the script take the most complete header available. Just an FYI if somebody else runs into a similar problem!

  • Geraldine_VdAuweraGeraldine_VdAuwera Cambridge, MAMember, Administrator, Broadie admin

    FYI, the behavior of CatVariants regarding headers is documented in the tool doc.

    More importantly, this tool is not appropriate for the use you're making of it. As noted above, the tool expects that the input VCFs represent non-overlapping intervals. The way you're using it, that expectation is not satisfied and the output vcf will most probably not be sorted correctly. You should be using CombineVariants for this.

Sign In or Register to comment.