Find single mutation with unified genotyper
Hi, I try to find a single mutation with unified genotyper but can't find it. In my sample (human gene SOD1 from chr21) I removed the stop codon by 2 deletions. Than i run UnifiedeGenotyper as follows:
java -Xmx4g -jar ../GenomeAnalysisTK-2.5-2-gf57256b/GenomeAnalysisTK.jar -T UnifiedGenotyper -R ../human_g1k_v37.fasta -I test/chr21_SOD1_ENSG00000142168_removedStop_DEL_2_at_33040889.bwa.bam -o test/chr21_SOD1_ENSG00000142168_removedStop_DEL_2_at_33040889.bwa.raw.indels.vcf -D ../dbsnp_137.b37.vcf -nt 2 -glm INDEL -stand_emit_conf 10 -stand_call_conf 10 -deletions -1 -L test.intervals.bed
The resulting .vcf-file contains only the header but no results.
I played with the following parameters, but with no success.
--indelGapOpenPenalty 5 -minIndelFrac 0 --min_indel_count_for_genotyping 1 -dt NONE --genotyping_mode GENOTYPE_GIVEN_ALLELES --output_mode EMIT_ALL_SITES
What could I do to find my mutation?