Heads up:
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
Notice:
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!

Test-drive the GATK tools and Best Practices pipelines on Terra


Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.

Mutect Analysis without a paired tumour normal & Analysis Criteria

ron128ron128 Member

Hello everybody, I am dealing with a dataset of cancer tumours sequenced on illumina hiseq 1000. I do not have a matched normal, and I have used Mutect to call somatic variants. I have the following doubts:

1) How good is mutect at calling variants, when there is no matched normal supplied? Should it even be used for this purpose?

2) On what basis is this criteria decided? Will i loose out on a lot of quality variants, if i discount all the variants marked as "REJECT" by mutect, and proceed ahead with only variants marked as "KEEP" in my downstream analysis? Alternately, will keeping all the "REJECTS" (which pass a certain mutant allele depth criteria) increase my false positives? Is the judgement criteria i.e. "KEEP" or "REJECT" an absolute criteria & to be strictly followed, or are there other parameters which I should look at as well? I am specifically asking in context of an analysis where there is no matched tumor normal. I would love to hear the communities thoughts on this subject.

Thanks a lot for your 2 cents!
Rohan.

Comments

Sign In or Register to comment.