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MuTect2 Tumour/Normal variant calling with only tumour samples

ISmoliczISmolicz United KingdomMember
Hello,
If two tumour samples are available from the same individual but no normal tissue e.g. primary and relapsed tumour, is it acceptable to use MuTect2 Tumour/Normal variant calling and treat the primary tumour sample as "normal" and relapsed tumour as "tumour" in the analysis?
Thank you for your help.
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