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AddOrReplaceReadGroups error even when all details are provided
[[email protected] S288C]$ java -jar $base_path/softwares/picard.jar AddOrReplaceReadGroups I=sortsam/$i_aligned_sorted_temp.bam O=sortsam/$i_aligned_sorted.bam RGID=$i RGLB=lib RGPL=illumina RGPU=unit1 RGSM=$i
INFO 2019-09-16 12:53:36 AddOrReplaceReadGroups
********** NOTE: Picard's command line syntax is changing.
********** For more information, please see:
********** The command line looks like this in the new syntax:
********** AddOrReplaceReadGroups -I sortsam/_aligned_sorted_temp.bam -O sortsam/_aligned_sorted.bam -RGID -RGLB lib -RGPL illumina -RGPU unit1 -RGSM
ERROR: Option 'RGLB' is required.
USAGE: AddOrReplaceReadGroups [options]
Assigns all the reads in a file to a single new read-group.
This tool accepts INPUT BAM and SAM files or URLs from the Global Alliance for Genomics and Health (GA4GH)
java -jar picard.jar AddOrReplaceReadGroups \
I have already added RGLB but still its showing error that RGLB is required