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How does the insert size influence the variant calling?

JulsJuls Member ✭✭

Hi,

@AdelaideR

I would like to use a public dataset that has been used for assembly originally for variant calling. All libraries come from the same sample and I define appropriate RG. However, there the libraries have varying high insert size (up to around 800). But all are paired end Illumina reads. Is this a problem? Should I exclude some?

Thanks!

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