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lluminaBasecallsToSam READ_STRUCTURE cannot recognize an index including only molecular barcodes

bhanuGandhambhanuGandham Cambridge MAMember, Administrator, Broadie, Moderator admin
edited April 23 in Ask the GATK team

Tool(s): IlluminaBasecallsToSam READ_STRUCTURE

Version(s): Latest public release version [2.19.0]

Description: READ_STRUCTURE=100T8B10M100T results the following error:
"Exception in thread "main" picard.PicardException: Column label (BARCODE_2) unrecognized. Library params(barcode params) can only contain the columns (OUTPUT, LIBRARY_NAME, SAMPLE_ALIAS, BARCODE, BARCODE_ where X is a positive integer) OR two letter RG tags!"
I wanted to describe "8bp index1 and 10bp index2". According to picard/src/main/java/picard/illumina/parser/ (a switch in line 142-157), the number of indices are counted by B. If READ_STRUCTURE=100T8B0B10M100T (using 0B10M to describe index2 without sample-barcode) is accepted, I can describe my read structure in READ_STRUCTURE.

Best Answer


  • bhanuGandhambhanuGandham Cambridge MAMember, Administrator, Broadie, Moderator admin
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