Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
poly-G tail trimming
As explained by qcfail, illumina's 2-color chemistry, which is used by HiSeq 4000, HiSeq X and NovaSeq, may produce false high-confidence poly-g tail in fastq reads.
Here is my question: is it necessary to do poly-G tail trimming before executing the germline pipelines of GATK?