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Using UG with a pol of 144 haploids
Dear GATK team,
I am trying to run the UG for 2 samples, each is a pool of 144 yeast strains (~200M reads for the 2 samples).
The run gets stuck after ~40,000 bases. My guess that using ploidy of 144 is too heavy, and cannot proceed.
I thought to split the run using the -L option to (lots of) multiple files along the chromosome, and run UG for each separately and merge. If this will work, it will also take a very long time to run. Any suggestions how to overcome this?