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error using somatic indel detector

EarendilEarendil Member
edited January 2013 in Ask the GATK team

hello every one please i got this error while running this command

[email protected]:~/analysis of rnaseq bamfiles$ java -jar GenomeAnalysisTK.jar -R VitisVinifera.fasta -T SomaticIndelDetector -o indels.vcf -verbose indels.txt -I:normal wt.bam -I:tumor newmut.bam

MESSAGE: SAM/BAM file SAMFileReader{/home/elendin/analysis of rnaseq bamfiles/newmut.bam} is malformed: Read HWI-ST132_0461:3:2201:1211:140854#GTCCTA is either missing the read group or its read group is not defined in the BAM header, both of which are required by the GATK. Please use http://www.broadinstitute.org/gsa/wiki/index.php/ReplaceReadGroups to fix this problem

ERROR ------------------------------------------------------------------------------------------

how can i add the missing read group for HWI-ST132_0461:3:2201:1211:140854#GTCCTA or define it in the header.

Post edited by Geraldine_VdAuwera on


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