If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!

Test-drive the GATK tools and Best Practices pipelines on Terra

Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
We will be out of the office on November 11th and 13th 2019, due to the U.S. holiday(Veteran's day) and due to a team event(Nov 13th). We will return to monitoring the GATK forum on November 12th and 14th respectively. Thank you for your patience.

Variant analysis on 1 or 2 samples: should I skip the final steps?

kennethcondonkennethcondon Member
edited June 2018 in Ask the GATK team

Hello all,

I'm a bit confused as to what steps are necessary, and what steps are not going to add much benefit. I have 2 jobs to complete for 2 different research groups we support: 1) Germline short variant discovery on whole exome sequencing (WES) data collected from 1 mouse (1 sample in total), and 2) Germline short variant discovery on whole genome sequencing data (WGS) collected from 2 macaques (2 samples in total).

I have written a wrapper that follows the GATK best practices from fastq preprocessing to HaplotypeCaller with appropriate conditional loops and required files specific to each species and type of sequencing.

According to the GATK workflow - my next steps after running HaplotypeCaller (with --emit-ref-confidence GVCF) in the pipeline are 1) consolidate GVCFs, 2) Joint-calling cohort, and 3) VQSR).

So here are my concerns:

  1. Considering I have only 1 or 2 samples - is it pointless doing some/all of these steps? Should I just stick to the variants called in each sample by HaplotypeCaller? Should I remove "--emit-ref-confidence GVCF" and just create a regular VCF? Is it possible to hard filter a regular VCF?
  2. If I do VQSR, I don't know where to find truth sets. Someone has suggested to me that I can use the human truth set in other species because the information taken from the truth set is the profile of what a true SNP looks like, not the position of the SNP - I'm really not sure about this.

I have previously posted this on biostars without success.

Help Me, Obi-Wan.

Thanks in advance.


Sign In or Register to comment.