Test-drive the GATK tools and Best Practices pipelines on Terra
Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
Picard tool CleanSam fails when using GCS paths
I tried to run CleanSam using both gatk220.127.116.11 and picardcloud.jar, and got the following error in both cases:
Exception in thread "main" htsjdk.samtools.SAMException: Cannot read non-existent file: file:///Users/rfrazer/Projects/moap/testing_staging_area/gs:/5aa919de-0aa0-43ec-9ec3-288481102b6d/tcga/OV/WGA_RepliG/WXS/BI/ILLUMINA/TCGA_MC3.TCGA-04-1335-11A-01W-0489-09.bam
The tool tried to look for the provided path in my current directory instead of trying to access GCS.
It was mentioned to me that not all Picard tools work with NIO right now, but all walker tools should. Since this tool is indeed a walker tool, I think this problem is worth looking into.
Also, is it possible to provide me with a list of Picard tools that should be able to work with NIO in GATK4? This will save my team a lot of time.