Ensembl GRCh38 indels and dsnp vcf files

samuelriverosamuelrivero Washington DCMember
edited May 2018 in Ask the GATK team

Hello,

I am using GATK3.8 to call variants in RNA-seq data. I am following the best practices for RNA-seq (https://software.broadinstitute.org/gatk/documentation/article.php?id=3891). My problem is during the indel realignment and base calibration steps. I have used the Ensembl GRCh38 genome version to map the reads. The indels and dsnp vcf files I can download from the resource bundle (hg38) have different chromosome notation and contig names (In my bam file chromosomes contain just number, ie "1", and in the vcf the format is "chr1"). Is there any indels and dsnp vcf files with the chromosome notation for Ensembl ? I can change the chromosome format in the vcf files but I dont know if this can be a problem in the next steps of the workflow.

Thanks

Sam

Post edited by samuelrivero on

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Answers

  • samuelriverosamuelrivero Washington DCMember

    I have downloaded them from Ensembl , but I am having issues because in some lines the ALT field is empty. I will remap my samples using your hg38 reference.

    Thanks

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