Heads up:
We’re moving the GATK website, docs and forum to a new platform. Read the full story and breakdown of key changes on this blog.
Notice:
If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!

Test-drive the GATK tools and Best Practices pipelines on Terra


Check out this blog post to learn how you can get started with GATK and try out the pipelines in preconfigured workspaces (with a user-friendly interface!) without having to install anything.
Attention:
We will be out of the office for a Broad Institute event from Dec 10th to Dec 11th 2019. We will be back to monitor the GATK forum on Dec 12th 2019. In the meantime we encourage you to help out other community members with their queries.
Thank you for your patience!

TruSeq Libraries influence in PON

laura_TLlaura_TL SpainMember

Hi,

We are trying to build a PON to use with MuTect2 GATK4. Our samples are sequenced using the library TruSeq Nano DNA 350. How important would be that the samples of the PON have exactly the same library? Would be fine if they were sequenced with TruSeq DNA PCR-free or with TruSeq DNA? We are trying to build it using samples from SRA, and there is just one sample from a healthy individual using our configuration (Illumina HiSeq 2x150 TruSeq NanoDNA350).

Thank you very much,

Tamara and Laura

Best Answer

Answers

Sign In or Register to comment.