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Test-drive the GATK tools and Best Practices pipelines on Terra
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Create a PoN from samples with different technical backgorund?
I read on your somatic mutation variant calling tutorial that even unmatched PoN could help for detecting sequencing artifacts. But the normal samples in my laboratory came from different exome enrichment kit (Illumina and Agilent) and from different sequencing platform (ION and Illumina). Would you recommend to use the normal samples from the same technical background? or Could we use the al the normal samples for our PoN?