Mutect2 PON with one normal sample
I am trying to analyze several tumors and one normal with mutect2. They are not paired. Is it appropriate to use -I normal ? or create pon with one normal sample and use -pon option? Tried to use mutect2 tumor-only mode to call the normal sample and use CreateSomaticPanelOfNormals, the result is an empty vcf with only headers. Any suggestions would be appreciated.